Pulsed electromagnetic fields ameliorate skeletal deterioration in bone mass, microarchitecture and strength by enhancing canonical Wnt signaling-mediated bone formation in rats with spinal cord injury.
pubmed: wnt1 2020-10-29
Summary:
Pulsed electromagnetic fields ameliorate skeletal deterioration in bone mass, microarchitecture and strength by enhancing canonical Wnt signaling-mediated bone formation in rats with spinal cord injury.
J Neurotrauma. 2020 Oct 27;:
Authors: Shao X, Yan Z, Wang D, Yang Y, Ding Y, Luo E, Jing D, Cai J
Abstract Spinal cord injury (SCI) leads to extensive bone loss and high incidence of low-energy fractures. Pulsed electromagnetic fields (PEMF) treatment, as a non-invasive biophysical technique, has proven to be efficient in promoting osteogenesis. However, the potential osteo-protective effect and mechanism of PEMF on SCI-related bone deterioration remain unknown. The spinal cord of rats was transected at vertebral level T12 to induce SCI. Thirty rats were assigned to the control, SCI and SCI+PEMF groups (n=10). One week after surgery, the SCI+PEMF rats were subjected to PEMF (2.0 mT, 15 Hz, 2 h/day) for 8 weeks. Micro-CT results showed that PEMF significantly ameliorated trabecular and cortical bone microarchitecture deterioration induced by SCI. Three-point bending and nanoindentation assays revealed that PEMF significantly improved bone's mechanical properties in SCI rats. Serum biomarker and bone histomorphometric analyses demonstrated that PEMF enhanced bone formation, as evidenced by significant increase in serum osteocalcin and P1NP, mineral apposition rate, and osteoblast number on bone surface. However, PEMF had no impact upon serum bone-resorbing cytokines (TRACP 5b and CTX-1) or osteoclast number on bone surface. PEMF also attenuated SCI-induced negative changes in osteocyte morphology and osteocyte survival. Moreover, PEMF significantly increased skeletal expression of canonical Wnt ligands (Wnt1 and Wnt10b) and stimulated their downstream p-GSK3β and β-catenin expression in SCI rats. This study demonstrates that PEMF can mitigate the detrimental consequence of SCI on bone quantity/quality, which might be associated with canonical Wnt signaling-mediated bone formation, and reveals that PEMF may be a promising biophysical approach for resisting osteopenia/osteoporosis following SCI in clinics.
PMID: 33108939 [PubMed - as supplied by publisher]