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Allostery is pertinent to regulate a protein’s functional activity induced by the action of an effector at a site distinct from the active site of biomolecules through alteration of conformation and/or dynamics. Allosteric Database (ASD) provides a versatile resource for structure, function, disease and related annotation for the well-established allosteric macromolecules and allosteric modulators since 1901, data in ASD is annually updated and freely served for biologists and medicinal chemists interested in allosteric regulation mechanism and allosteric drug discovery.