An in vivo electrophysiological preparation for mechanical, electrical and optical stimulation of sensory neurons that innervate murine bone

In this study, we aimed to develop an in vivo electrophysiological bone-nerve preparation to record the activity of peripheral sensory neurons that innervate the murine tibia. A small nerve that innervates the tibial marrow cavity was identified in isoflurane-anesthetized C57BL/6 mice, and placed over a platinum hook electrode for extracellular recording. Whole-nerve activity was amplified, filtered and sampled at 20 kHz using PowerLab (ADInstruments). A cannula was placed into the marrow cavity...