NH4Cl affects the expression of Wnt/β-catenin pathway in hepatocytes.
pubmed: wnt1 2017-10-05
Summary:
NH4Cl affects the expression of Wnt/β-catenin pathway in hepatocytes.
Can J Physiol Pharmacol. 2017 Oct 04;:
Authors: Bai L, Li J, Liu X, Li S, Li F, Chen Y, Yu Z
Abstract We intend to explore whether NH4Cl influences the viability and regulates the expression of Wnt/β-catenin pathway in hepatocytes.The Chang liver cell line was used and culture with different concentrations of NH4Cl (2.5, 5, 10, 20, 40 and 50mM/l) for 12h, 24h and 48h, respectively. The viability of hepatocytes was detected by MTT assay. The mRNA and protein expression level was analyzed with qRT-PCR and Western blotting, respectively. NH4Cl concentration significantly affects the viability of hepatocytes. With the increase of NH4Cl concentration, the viability of hepatocytes was decreased, accordingly. Totally, the mRNA and protein expression of Wnt1, β-catenin and cycling D was significantly increased after treatment with low concentrations of NH4Cl as compared to the control group, whereas their expression levels were decreased after treatment with high concentrations of NH4Cl. The mRNA and protein expression of Wnt1, β-catenin and cycling D was also significantly increased after treatment with NH4Cl for short period as compared to the control group, whereas their expression levels were decreased after treatment with NH4Cl for long period. Besides, we found NH4Cl treatment significantly reversed the results after RNA silencing of Wnt1 in hepatocytes.NH4Cl influences the viability of hepatocytes and affects the expression of Wnt/β-catenin pathway in hepatocytes.
PMID: 28977758 [PubMed - as supplied by publisher]