Comparative transcriptome analysis reveals conserved branching morphogenesis related genes involved in chamber formation of catfish swimbladder.
pubmed: wnt1 2017-11-24
Summary:
Comparative transcriptome analysis reveals conserved branching morphogenesis related genes involved in chamber formation of catfish swimbladder.
Physiol Genomics. 2017 Nov 22;:physiolgenomics.00089.2017
Authors: Yang Y, Fu Q, Liu Y, Wang X, Dunham R, Liu S, Bao L, Zeng Q, Zhou T, Li N, Qin Z, Jiang C, Gao D, Liu Z
Abstract The swimbladder is an internal gas-filled organ in teleosts. Its major function is to regulate buoyancy. Swimbladder exhibits great variations in size, shape, number of compartments or chambers among teleosts. However, the genomic control of swimbladder variations is unknown. Channel catfish (Ictalurus punctatus), blue catfish (Ictalurus furcatus), and their F1 hybrids of female channel catfish x male blue catfish (C×B hybrid catfish) provide a good model to investigate the swimbladder morphology, because channel catfish possess a single-chambered swimbladder whereas blue catfish possess a bi-chambered swimbladder; and C×B hybrid catfish possess a bi-chambered swimbladder but with significantly reduced posterior chamber. Here we determined the transcriptional profiles of swimbladder from channel catfish, blue catfish, and C×B hybrid catfish. We examined their transcriptomes at both the fingerling and adult stages. Through comparative transcriptome analysis, approximately 4,000 differentially expressed genes (DEGs) were identified. Among these DEGs, members of the Wnt signaling pathway (wnt1, wnt2, nfatc1, rac2), Hedgehog signaling pathway (shh), and growth factors (fgf10, igf-1) were identified. As these genes were known to be important for branching morphogenesis of mammalian lung and of mammary glands, their association with budding of posterior chamber primordium and progressive development of bi-chambered swimbladder in fish suggested that these branching morphogenesis related genes and their functions in branching are evolutionarily conserved across a broad spectrum of species.
PMID: 29167198 [PubMed - as supplied by publisher]