Optimal CCN4 Immunofluorescence for Tissue Microarray
pubmed: wnt1 2023-01-28
Methods Mol Biol. 2023;2582:13-21. doi: 10.1007/978-1-0716-2744-0_2.
CCN4 (also known as WNT1-Inducible Signaling Pathway Protein 1 or WISP1) is a 367 amino acid, 40 kDa protein located on chromosome 8q24.1-8q24.3. Prior studies have provided support for a pro-inflammatory role for CCN4. We have shown recently that CCN4 expression is associated with advanced disease, epithelial-mesenchymal transition, and an inflamed tumor microenvironment in multiple solid tumors. We detail here the CCN4 tissue microarray immunofluorescence protocol related to these findings.