Co-CRISPR: A valuable toolkit for mutation enrichment in the gene editing of Spodoptera frugiperda

pubmed: wnt1 2023-01-28

Summary:

The CRISPR/Cas9 system has been successfully applied in dozens of diverse species; although the screening of successful CRISPR/Cas9 editing events remains particularly laborious, especially for those that occur at relatively low frequency. Recently, a co-CRISPR strategy was proved to enrich the desired CRISPR events. Here, the co-CRISPR strategy was developed in the Fall armyworm, Spodoptera frugiperda, with kynurenine 3-monooxygenase gene (kmo) as a marker. The kmo mosaics induced by...

Link:

https://pubmed.ncbi.nlm.nih.gov/36169087/?utm_source=Other&utm_medium=rss&utm_campaign=None&utm_content=16uwQpOeqFYN8R4TKOtwPy2utpqy9ex2oldalD2yF_fQHv2caq&fc=None&ff=20230128204931&v=2.17.9.post6+86293ac

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Exome ยป pubmed: wnt1

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Authors:

Wei-Kang Han, Feng-Xian Tang, Hao-Li Gao, Yan Wang, Na Yu, Jian-Jun Jiang, Ze-Wen Liu

Date tagged:

01/28/2023, 20:49

Date published:

09/28/2022, 06:00