MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila

Synth Syst Biotechnol. 2025 Apr 3;10(3):783-793. doi: 10.1016/j.synbio.2025.04.001. eCollection 2025 Sep.

ABSTRACT

Nowadays, biological databases are playing an increasingly critical role in biological research. Myceliophthora thermophila is an excellent thermophilic fungal chassis for industrial enzyme production and plant biomass-based chemical synthesis. The lack of a dedicated public database has made access to and reanalysis of M. thermophila data difficult. To bridge this gap, we developed MTD (https://mtd.biodesign.ac.cn/), a cloud-based omics database and interactive platform for M. thermophila. MTD integrates comprehensive genome annotations, sequence-based predictions, transcriptome data, curated experimental descriptions, and bioinformatics analysis tools, offering a comprehensive, one-stop solution with a 'top-down' search strategy to streamline M. thermophila research. The platform supports data reproduction, rapid querying, and in-depth mining of existing transcriptome datasets. Based on analyses using data and tools in MTD, we identified shifts in metabolic allocation in a glucoamylase hyperproduction strain of M. thermophila, highlighting changes in fatty acid biosynthesis and amino acids biosynthesis pathways, which provide new insights into the underlying phenotypic alterations. As a pioneering resource, MTD marks a key advancement in M. thermophila research and sets the model for developing similar databases for other species.

PMID:40276250 | PMC:PMC12018684 | DOI:10.1016/j.synbio.2025.04.001