Integrating metabolite-based molecular networking with database matching and LC-MS-guided targeted isolation for the discovery of novel chemical constituents: application to Euphorbia helioscopia L

Molecular networking (MN) analysis facilitates the targeted discovery of novel constituents and enhances the understanding of natural products. While various molecular networks could reduce the effects of redundant nodes, current research is still limited by the interference from the same and coeluted metabolites, including isotopic peaks, a variety of adduct ions, in-source fragmentations, and dehydration. This research proposes a novel strategy: stratified precursor lists (SPLs)-guided...