Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)
Latest Articles from Biodiversity Data Journal 2022-06-30
Summary:
Biodiversity Data Journal 10: e84860
DOI: 10.3897/BDJ.10.e84860
Authors: Cristina Vasilita, Marina Moser, Lars Krogmann
Abstract: DNA barcodes provide a reliable and efficient solution to resolving cryptic species complexes and accelerate species discoveries. The superfamily Ceraphronoidea (Hymenoptera) is a group of parasitoid wasps for which a barcoding approach could be of great help, if it were not for the very poor results. The inability to obtain barcodes for the majority of treated ceraphronoids halts progress on the taxonomy of this hyperdiverse parasitoid group. We here present a working protocol for the barcoding of ceraphronoid wasps which yields a first-time over 90% success rate.